Sending Samples for preliminary
Work-up or clinical PGD
encourage you to contact us at any time with questions or
concerns while preparing blood samples, preparing for
blastomere biopsy or during the biopsy.
Remember, PGD for single gene diserders is quite different
from analysis for chromosomes, where DNA contamination is
not a problem. Because of being a DNA based procedure,
contamination can cause a misdiagnosis. The biopsy technique
used at your IVF center should be evaluated to assure that a
result can be obtained and contamination is not introduced
into the specimen.
Before sending your first case, schedule
a sample trial that we can analyze. We will provide feedback
and suggestions for improved technique when indicated.
The method of entering the zona pelucida has not been shown
to affect the analysis, slit, acid Tyrodes, or laser are all
acceptable. It is important to visually confirm that an
intact blastomer has been placed into the lyses buffer.
If the blastomer is disrupted during biopsy, wash or
transfer into lyses buffer a second blastomer should be
biopsied, placed in a separate tube and labeled (as a second
cell). The embryo should be marked as possibly contaminated.
Whenever you have a question or concern, do not hesitate to
Guidelines for transport PGD