When the oocytes are ready to be retrieved, another vaginal
ultrasound similar to those performed to monitor follicular
development is performed.
A small needle inserted in a guide attached to the
ultrasonic probe is directed through the vaginal wall into
the ovarian follicles.
The needle is connected to a suction pump and the fluid from
each accessible ovarian follicle within the ovary is
The aspirated liquid is transferred to the laboratory and
examined microscopically to recover the eggs which will be
immediately placed in a nutrient broth.
Fertilization and Embryo Culture